How can I identify the Fusarium culmorum with molecular technology?

More Vipin Panwar's questions See All

Total phenol content estimation?

The addition of Na2CO3 in TPC estimation creates an alkaline medium for phenols to get unstable, however whenever I add 2 ml of 20% of Na2CO3 to 1 ml of gallic acid extract and 0.5 ml of FC...

14 May 2024 5,478 2 View

Is creating conducive environment for in service teacher professional development important? If we agree to this how to create it?

Importance of creating conducive environment for better learning of students in school as well as at home has been highly recognised by educationist across all corners of the globe. Is such an...

26 April 2024 1,662 12 View

Validation of the formula for beta-carotene estimation?

For the estimation of beta-carotene by acetone extraction method using petroleum ether and sodium sulphate at 453 nm. it is known to all that the extinction coefficient of beta-carotene at 453 nm...

16 April 2024 3,739 1 View

Can Anyone Explains the absence of reduction peak at 0.8V in CV of Pt/C?

Hello All, Recently I obtained a CV of Pt/C attached on GC using 0.25%Nafion as binder in 0.5M H2SO4 (N2 saturated) at 0.01V/S rate. The reference i used was Ag/AgCl. Unfortunately, the peak shape...

10 April 2024 8,958 2 View

What can be the potential research questions for investigation in school education?

The quality of school education needs special attention for developing a better society where the human beings, other creatures and the eco system are safe for us and our future generations. To...

19 March 2024 7,176 3 View

How to convert ppm to ml for photocatalysis hydrogen evolution reaction?

Gas PPM= 200 ppm for 1 ml syringe Headspace Volume = 40 ml. GC has been calibrated with in PPM units.

21 December 2023 7,068 1 View

Can we deploy resource provisioning model using machine learning approach on AWS but on which one to deploy AWS Sagemaker,Terraform,or Pipelines etc?

I am a Ph.D. scholar working in cloud resource provisioning frameworks using a machine learning approach for service-based cloud applications(using java) want to deploy a model on a public cloud...

24 August 2023 269 2 View

How to make table and report result of repeated measure ANOVA in APA?

Dear ResearchGate Community, I am seeking guidance regarding the appropriate statistical analysis for my research study. In my study, I have two groups (Control and Experimental) and two states...

05 June 2023 3,858 4 View

Can a immiscible polymer blend in solution form become miscible in solid form after removal of the solvent?

22 May 2023 8,909 3 View

Is band gap depend on the number of unit cells?

Dear All, How does the band gap depend on the number of unit cells? From the computational study, I found that the band gap size of a 2D crystalline solid depends on the number of unit cells....

01 March 2023 7,951 3 View

Hello Researchers, Can anyone guide me on estimating the Leaf Area Index (LAI) of wheat using only weather and soil parameters? Is it possible?

I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?

28 July 2024 4,886 2 View

Markers to distinguish individual cardiomyocytes?

I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...

20 June 2024 3,225 2 View

How top dressing of nitrogenous fertilizers can be done in mulched wheat and rice crops and how should FYM/compost be applied in zero till wheat?

02 June 2024 3,836 3 View

What is the size of EF-1-alpha for Fusarium spp. ?

The primers size

29 May 2024 772 1 View

Gametocide for wheat?

We need to produce hybrid wheat seed for our experiment. Ca anyone tell me what's the best chemical agent for this purpose and where to buy the chemical agent? Thank you for your help.

27 May 2024 834 2 View

What are the essential data and calculations to be taken for the research that aims to increase soil Organic matter using mungbean as cover crop?

I am a researcher of Soil science department. 5 months ago I had planted wheat for my research. The research design was split plot design. it has 3 replications, each replication has 2 main plot...

05 May 2024 4,815 3 View

How can I extract DNA using Chelex100 from wheat common bunt?

Hi everyone, I'm trying to extract fungal DNA from wheat common bunt using chelex100 and I've tried several different protocols without any success. I've used different concentrations of chelex100...

29 April 2024 7,317 1 View

If the seeds didn't germinate well equally on every plot and i need data from 1m2 area only. can i sow seeds in 1m2 in gap filling for research?

I am a researcher of Soil science department. 5 months ago I had planted wheat for my research. The research design was split plot design. it has 3 replications, each replication has 2 main plot...

28 April 2024 7,432 5 View

How to submit coding sequences in genebank?

Hii all.. I have few sequences of Fusarium spp partial TEF region. I want to submit them to genebank to get accession numbers. Can someone please help how to submit sequences of coding regions? It...

23 April 2024 2,468 6 View

Which will be a more higher expression between the TrpC promoter and TEF1 promoter in Fusarium graminearum?

Now, I am working on a overexpression experiment of a fungus gene using the pFA6-kanmx4 vector with TEF1 promoter and terminator on geneticin(G418) cassette, but it doesn't work well. Because I...

17 April 2024 3,651 0 View

Adam Okorski

Vipin for quantitative detection You can use qPCR method, for qualitative analysis I recommend the use of SCAR PCR or Nested PCR.

Best regards

qPCR with Sybr Green: http://www.ncbi.nlm.nih.gov/pubmed/19047000

If You need for more information take a look to https://ediss.uni-goettingen.de/bitstream/handle/11858/00-1735-0000-000D-F220-2/brandfass.pdf?sequence=1

Vipin Panwar

Thanks Adam.

Oadi Matny

You can use specific - specific primer design for F. culmorum.