Hii all.. I have few sequences of Fusarium spp partial TEF region. I want to submit them to genebank to get accession numbers. Can someone please help how to submit sequences of coding regions? It is asking for corresponding amino acid sequence. I derived that. But after submitting the amino acid sequences, it is saying there are internal stop codons (I had deleted the stop codons denoted by "hyphen"). What app or methods should I use?
Helllo,
Submitting genes/partial genes in a genebank can be tricky sometimes. A good starting point would be to do a blast of your sequence in the genebank, and using the hit has guidance. Essentially, see how the closer related genes to yours were submitted.
Bhavya G. , I found the solution thanks to the NCBI team. You actually have to blast your coding sequence and get exon locations by comparing them with sequences submitted previously. Then you can either make a feature table indicating the exon regions; or make an amino acid FASTA file by joining the exon translations. I am attaching files shared by NCBI team. You can contact [email protected] for assistance.
