I am getting smear while running a gel from PCR amplification. I believe it is due to non specific amplification of primer because i have already tried all the technical trouble shooting methods so far.
Try to get the non-specific amplicon by reducing the annealing temparature and extract from gel , purify and send for sequencing or clone into a vector by TA cloning and send for sequencing. But this method does not solve your problem. I suggest you to design new primer pair and repeat the PCR.