i have been performing quantitative phytochemical analysis for past week and have found it really difficult to understand how to set a specific concentration of sample for conducting the experiments. i have been thinking about taking very low concentrations of sample like 10-50 micro liters. but here the practical difficulty is that most of the time color is not getting developed for specific tests and it is difficult to take OD value in spectrophotometer. what to do?
Here are some suggestions to address this issue:
Optimize the sample concentration:
It is important to optimize the sample concentration for each specific test to ensure that the results are accurate and reproducible. This can involve testing different concentrations of the sample to determine the optimal concentration for each test.
Increase the sample volume:
Increasing the sample volume can help to increase the concentration of the sample and make it easier to develop color and take OD values. However, it is important to ensure that the sample volume is not too high, as this can lead to interference from other compounds in the sample.
Use a different method:
If the current method is not working, it may be necessary to try a different method for the specific test. For example, some tests may require a different solvent or a different pH to develop color.
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