How can i identify non specific sites of DNA where primers are binding?

I am getting smear while running a gel from PCR amplification. I believe it is due to non specific amplification of primer because i have already tried all the technical trouble shooting methods...

17 June 2018 9,070 3 View

Did anyone try the Mk 1 MinION?

I want to know if it is working as advertised. How many reads am I to expect in realistic settings? How bad is the quality?

07 January 2016 1,917 1 View

Is there a preferred way to use projection mapping for the world?

I am doing a research paper on cartography. I know that there are different opinions regarding the most useful type of projection map. I'm just looking for some opinions and why you think, for...

22 September 2015 8,000 14 View

How do you determine whether a news source is reputable? Or a news story is reliable?

We are thinking through some of the problems in distinguishing reputable news from phony ones. What might give you a clue that an online story you are reading is bogus, fake, or unreliable? We'd...

20 May 2015 6,012 41 View

Can feces can have traces of hormones, which can give clues about oestrus?

At least in the case of higher mammals

17 June 2014 862 13 View

How would you access news (or any other information) in a language you don't know?

Say, you want to know what the Russian and Ukrainian online news are saying about the recent Kiev events and the EU Summit? Or, say, you'd like to see first hand what the Chinese or Malaysian...

27 March 2014 1,395 28 View

Assuming that most data (big or not) tends to have errors, what about data quality and veracity? Is your Big Data messy?

What would you suggest for big data cleansing techniques? Is your big data messy? If so, how (or how much) does it affect your work / research? How do you get rid of noise? How do you verify big...

13 January 2014 1,799 17 View

Is anyone working on Personalized (precision) cancer medicine?

We are working on a new wiki for precision cancer medicine. If you are working in the field, please let me know what you think (registration is required): http://rubinlab.bgu.ac.il/pcm/

19 August 2013 260 2 View

How to compare the quality of clustering solution for the same dataset?

I always wondered how to test for the significance of clustering results. Now I have an urgent need. I want to show that when my input is organized in a biologically meaningful way. For that, I...

02 June 2012 828 15 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View