Prameela Awale

2 Questions 4 Answers 0 Followers

Questions related from Prameela Awale

How Blunt end ligation for self circulation of Genomic DNA be done succesfully?

Hello All. I am trying to ligate the blunt end of maize genomic DNA digested by restriction enzyme. I do not have the option of using restriction enzyme with sticky end cuts.. I am using T4 DNA...

01 October 2019 4,837 6 View

How can i identify non specific sites of DNA where primers are binding?

I am getting smear while running a gel from PCR amplification. I believe it is due to non specific amplification of primer because i have already tried all the technical trouble shooting methods...

18 June 2018 9,136 3 View