Prameela Awale

4 Questions 6 Answers 0 Followers

Questions related from Prameela Awale

How a Downstream Gene Variant affect the function of a gene?

I have Downstream gene variant as the type of variant in one of the gene of my interest affected by mutation. But now, I am having trouble interpreting how the sequences affected after the gene...

27 May 2020 1,492 0 View

How can smear in Genomic DNA can be reduced?

I use Qiagen Kit for DNA extraction and also tried couple other kits but every time I am getting lots of smear when I run the newly extracted DNA in gel. I also tried CTAB based extraction and It...

17 October 2019 9,091 4 View

How Blunt end ligation for self circulation of Genomic DNA be done succesfully?

Hello All. I am trying to ligate the blunt end of maize genomic DNA digested by restriction enzyme. I do not have the option of using restriction enzyme with sticky end cuts.. I am using T4 DNA...

01 October 2019 4,877 6 View

How can i identify non specific sites of DNA where primers are binding?

I am getting smear while running a gel from PCR amplification. I believe it is due to non specific amplification of primer because i have already tried all the technical trouble shooting methods...

18 June 2018 9,177 3 View