Hello,
I will do my co-culture THP-1 cells and HepG2 cells using Transwell. After the differentiation, I don't know how I can haverst my cells in the the insert of Transwell to do the FACS. Please share me your experiences. Thank you!
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View
What should be the frameworks or methodologies to examine written academic ELF? I want to explore the linguistic features of written ELF in research articles.
23 July 2024 4,800 1 View
Dear all, May I ask a question about indexed by Scopus. 1 month ago, I have published my paper in the Journal name: Challenges in Sustainability in 20/05/2024. However, this Journal has changed...
06 July 2024 6,734 2 View
Dear Colleagues, I am currently formulating oil in glycerin/water nanoemulsionn of Thymol and Eugenol, using cationic surfactant. After phase diagram, I got trouble that the clear and...
03 July 2024 4,200 2 View
How to calculate Module Young from compressive strength and strain curve
29 June 2024 817 3 View
I have been working in Hanoi University of Public Health. I would like to know where and how I can buy AMOS software?
28 June 2024 6,593 1 View
When choosing the receptor model (rigid or flexible) in molecular docking, does it depend on the designed ligands' characteristics or anything else? For instance: + With the ligand A, the protein...
25 June 2024 2,716 0 View
I prepare Mg doped Hydroxyapatite by wet preipitation but (Ca + Mg)/P ratio is higher than 1,82. CaO peak cannot be detected in XRD spectrum
23 June 2024 8,970 19 View
I follow the protocol in this paper "Computational protein–ligand docking and virtual drug screening with the AutoDock suite" for the "Flexible Docking". Everything is smooth until I write the...
22 June 2024 3,089 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View