Hello
If you mean the laboratories of cell lines you can purchase this cells line from Altogen Labs CRO or from Shanghai Institute of Cell Biology , then cultuer this cells in Minimum Essential Medium (DMEM and RPMI1640 also works well) supplemented with 10% FBS. Discard culture medium every 2-3 days . To passage cells, briefly rinse cell monolayer with 1xPBS twice and add pre-warmed (37°C) 0.05% Trypsin-EDTA solution for 5 – 7 minutes . Once cell layer is dispersed (5-7 min at 37°C) deactivate Trypsin by adding equal volume of complete growth medium with 10% FBS .To avoid clumping don’t agitate the cells by shaking the flask while waiting for the cells to detach .Split cells 1:4 every 3 days (recommended), or 1:8 every 6 days.Cultures should be incubated at 37°C in humidified atmoshere with 5% CO2
Good Luck
http://www.lgcstandards-atcc.org/products/all/HB-8065.aspx?geo_country=ro
http://www.hepg2.com/
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