Penicillium is a problem in Petri plates having PDA media. I have sterilized the PDA media but still, it is a potential threat. I want to work on other fungi but I face Penicillium and its spores. Kindly give me tips how to get rid of this notorious fungus.
Hello
Check the following :
A: If you see the contamination on non-inoculated petri dishes, the fungi are coming from inadequately sterilized media and/or apparatus. B: If the contamination is appearing at the points of inoculation (on plates) that mean the fungi are either in the inoculum or on the instruments that you are inoculating with. C: If the contamination is appearing on the surface of the plates, but away from the places that you inoculated the fungal spores are probably moving in from the airborne. Then after, we can find solution to this problem
Good luck
Mr. Abbas, I suggested that you can recheck the procedure and be highly aseptic in the operations especially during subculturing the isolates.
If it turns out that your problem is airborne contamination (as described by the troubleshooting analysis above), then you could try spraying a "mist" of 70% ethanol solution in the air around your work area, e.g. by having a spray-bottle filled with your 70% ethanol solution, and the nozzle adjusted to "fine spray". Spray alot of mist into the air over your workspace. Wait 5-10 minutes for the droplets to settle (try to limit the air movement in your workarea during this time. No fans or open windows). Then wipe down the bench with more 70% ethanol before starting your inoculations.
Muhammad Fahim: Good suggestions. Yes, I will follow these suggestions.Thanks
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