I am currently using Hela cells. I am trying to transfect them with a specific plasmid and then infect the with HSV-1. Unfortunately, all my transfected cells seems to not be infected.
It's like the transfection is blocking the infection process.
How could I overcome this issue.
Thank you
First make stable transfected cells... Use a marker to select and seperate your transfected cells ( may be by flowcytometry)... Then use those for infection with your virus
I want to make transient transfection.
I was wondering if there was another protocole to do this.
I’m agree with Prof. Diptiman Choudhury.
Maybe, you can try to use NEPA gene electroporation method to obtain stable transfected cells and further separated by flowcytometry and use those for infection with HSV-1.
Dear Mayerline,
your transgene plasmid should include some gene marker for instance GFP or some specific antibiotic resistance gene which help you to select your transfected cells and after that you will be able to start transduction process.
Best,
Niloufar
got exactly the same issue and still looking for an answer , maybe plasmids have CpG motif that stimulate innate immunity
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