Mayerline Dorsainvil

4 Questions 4 Answers 0 Followers

Questions related from Mayerline Dorsainvil

Is the effect of Bapta-AM reversible?

I use Bapta-Am to reduce the cytosolic calcium inside mammalian cells. I was wondering after how long the calcium level go back to normal and hide the effect of the Chelator. I use 10-20uM.

27 February 2019 602 1 View

How can i get Hela cells to be transfected and infected in the same cells?

I am currently using Hela cells. I am trying to transfect them with a specific plasmid and then infect the with HSV-1. Unfortunately, all my transfected cells seems to not be infected. It's...

31 May 2018 8,055 5 View

How can i get Hela cells to be transfected and infected in the same cells?

I am currently using Hela cells. I am trying to transfect them with a specific plasmid and then infect the with HSV-1. Unfortunately, all my transfected cells seems to not be infected. It's...

05 May 2018 6,821 4 View

My mutagenesis reaction doesn't seems to work. What can i do to increase the effeciency of the reaction?

I think it might be the fact that I put it at -20degree Celcius after the reaction. Can it be a cause of DNA degradation, should I keep it at 4degree Celcius instead?

17 April 2018 4,029 3 View