I use Bapta-Am to reduce the cytosolic calcium inside mammalian cells. I was wondering after how long the calcium level go back to normal and hide the effect of the Chelator. I use 10-20uM.
Sheng-Nan Wu
BAPTA-AM is membrane-permeable and can pass cell membrane into the cell interior. Once it is degraded by esterase(s), free form may bind to intracellular calcium. As a result, BAPTA should never come back to the ester form (i.e., BAPTA-AM). Hence intracellular cytosolic calcium should be low and difficult to come back normal once BAPTA is constantly trapped inside the cell. Therefore, the chelating effect of BAPTA-AM should be irreversible.
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