I have a sequence of my protein but I don't know whether it has a signal sequence for its extracellular secretion. If it does not, what signal sequence do I need to add for the secretion?
I think we'll need to know what cells you are working with. Secretion signal sequences can vary from species to species - have you had a look at what proteins are known to be secreted by your cells and what kind of signal peptides they carry? That would be a good place to start.
You can check its uniprot id and protein sequence in NCBI. The protein sequence there will tell you how many amino acid long is the proteins primary conformation. If the provided protein sequence is 16aa long than reported length of protein then u may have the signal sequence reported. but in that case the first aa will be Meth and 17 will be meth. rest should match your protein sequence.
This may not be the best explanation you expect but it has worked for me..
Gudluck
My protein is Bovine origin and it is secreted in rare occasions for that it needs some changes in the aa at particular point..i think i have to add a signal sequence to it so that it will be secreted out..i am using pVAX vector and tranfection into BHK-21 cells...
So you're expressing a bovine protein in BHK-21. You say it's rarely secreted, do you mean it's rarely secreted from BHK-21 cells or from the bovine cells the gene was clones from? I'm not familiar with mammalian cell lines, so my short and obvious best answer would be to look up what sequence(s) the BHK-21 cell line recognizes as a signal peptide and add it to your protein. Since your protein is rarely secreted, either te mechanism is different from a signal peptide, or different isoforms exist.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques