Abhinav Chaudhary

11 Questions 35 Answers 0 Followers

Questions related from Abhinav Chaudhary

How to prepare NALC buffer for liquefaction of non-TB target sputum?

Can anyone tell me if NALC-NaOH is suitable for liquefaction of sputum for real-time pcr amplification of non TB pathogenic bacteria and virus. Otherwise how to prepare NALC-NaOH or NALC PBS...

01 January 2019 2,756 0 View

Ho can I DNA extraction from tallow or beef fat??

Hi.. I need a suggestion to isolate animal DNA from tallow or beef fat.. Proceesed tallow doesnt allow recovery of DNA as it has been boiled over 110 deg celcius. Still i need a method to work...

12 December 2015 5,526 3 View

Two or three step annealing for low Tm probes in AT rich region?

I want to do to genotyping of the a sample and designed the probes with many softwares but all are giving me probes with tm below 59 deg. as per qPCR guidelines I tried making primers at 4-5 deg...

07 July 2014 5,404 1 View

TaqMan SNP genotyping: Is there a need to use the UNG activation step?

I am using TaqMan probes for SNP genotyping on LightCycler 480. I am facing some difficulties in resolution of heterozygous genotypes. The software can not identify it as " both alleles"...

11 November 2013 6,758 2 View

Do I need to activate UNG in Taqman Universal Master mix?

I am doing SNP genotyping on Lightcycler 480 using taqman probes and Taqman Universal Master Mix with UNG. Sometime the software get a low signal for heterozygous samples and the software is not...

11 November 2013 8,884 5 View

Any suggestions for genetic risk prediction?

I am trying to understand the implication of some SNPs responsible for disease in cattle. I want to calculate and predict the risk of disease from risk allele of an SNP in an individual animal...

11 November 2013 7,199 1 View

What is the optimum amount of plant tissue to get 1 µg of mitochondrial DNA?

I want to know what should be the starting amount of tissue to get at least 1 µg of pure mitochondrial DNA from plant seedlings? I know mito DNA is ~5% of total DNA but what yield is expected from...

06 June 2013 4,139 1 View

TB detection kit for Roche Light Cycler

Can anyone please suggest a kit for Mycobacterium tuberculosis detection on Roche Light Cycler 480 II?

04 April 2013 5,136 1 View

Blood parasite detection using PCR?

Can anyone tell me how long it is good to store animal blood without reducing the parasite detecting efficiency? I tried isolating whole blood DNA after 2 months of storage and it is not giving...

02 February 2013 2,494 5 View

Does anyone know a way to remove genomic DNA contamination from Mitochondrial pellet using DNAse treatment?

I am facing the problem of genomic DNA contamination in my mitochondrial DNA isolation from insect tissue. I want to include a DNAse treatment of my whole mitochondrial pellet before proceeding...

12 December 2012 5,876 7 View

Can anyone working on IonTorrent suggest a good way to reduce polyclonal ISPs?

I have run some chips (318) on Ion Torrent but I am getting high polyclonal beads many times. I have tried reducing my library concentration but it has not worked for me, can anybody suggest a...

11 November 2012 7,462 7 View