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Questions related from Abhinav Chaudhary
Can anyone tell me if NALC-NaOH is suitable for liquefaction of sputum for real-time pcr amplification of non TB pathogenic bacteria and virus. Otherwise how to prepare NALC-NaOH or NALC PBS...
01 January 2019 2,769 0 View
Hi.. I need a suggestion to isolate animal DNA from tallow or beef fat.. Proceesed tallow doesnt allow recovery of DNA as it has been boiled over 110 deg celcius. Still i need a method to work...
12 December 2015 5,537 3 View
I want to do to genotyping of the a sample and designed the probes with many softwares but all are giving me probes with tm below 59 deg. as per qPCR guidelines I tried making primers at 4-5 deg...
07 July 2014 5,414 1 View
I am using TaqMan probes for SNP genotyping on LightCycler 480. I am facing some difficulties in resolution of heterozygous genotypes. The software can not identify it as " both alleles"...
11 November 2013 6,770 2 View
I am doing SNP genotyping on Lightcycler 480 using taqman probes and Taqman Universal Master Mix with UNG. Sometime the software get a low signal for heterozygous samples and the software is not...
11 November 2013 8,895 5 View
I am trying to understand the implication of some SNPs responsible for disease in cattle. I want to calculate and predict the risk of disease from risk allele of an SNP in an individual animal...
11 November 2013 7,208 1 View
I want to know what should be the starting amount of tissue to get at least 1 µg of pure mitochondrial DNA from plant seedlings? I know mito DNA is ~5% of total DNA but what yield is expected from...
06 June 2013 4,148 1 View
Can anyone please suggest a kit for Mycobacterium tuberculosis detection on Roche Light Cycler 480 II?
04 April 2013 5,148 1 View
Can anyone tell me how long it is good to store animal blood without reducing the parasite detecting efficiency? I tried isolating whole blood DNA after 2 months of storage and it is not giving...
02 February 2013 2,504 5 View
I am facing the problem of genomic DNA contamination in my mitochondrial DNA isolation from insect tissue. I want to include a DNAse treatment of my whole mitochondrial pellet before proceeding...
12 December 2012 5,886 7 View
I have run some chips (318) on Ion Torrent but I am getting high polyclonal beads many times. I have tried reducing my library concentration but it has not worked for me, can anybody suggest a...
11 November 2012 7,472 7 View