Which packages can I use for meta-analysis of RNA-seq data and batch effect removal?

Dear All I have already collected RNA-seq expression profile of different studies issuing plant-microbe interaction. I have got read count files for different samples and genes. Now I want to...

14 January 2021 9,119 2 View

Explicit or implicit instruction: The trade-off between feasibility and productivity. Can a language be taught efficiently in an EFL setting?

Teaching realistically in an EFL setting would force us to rely on leaners' explicit knowledge and in turn explicit instruction. On the other hand, long hours of teaching explicitly may not lead...

17 December 2020 9,317 4 View

Aggregate distance in concrete?

I want to know if there is a function for aggregate distance in concrete?

06 October 2019 3,258 3 View

What is the reason for using unperforated region on tubesheet in Shell and tube condensers?

Hi everybody; I attached two picture of tubesheets of shell and tube condenser in which unperforated region are used in the middle of perforated region. I would be grateful if you can help me...

22 June 2019 3,920 3 View

Is there any Software to import HPLC data to PCA ?

Hello Is there any software to import HPLC data to PCA ?

29 April 2019 7,164 7 View

Can we use FACS Calibure flow cytometer in order to detect CD 51antibody with material number bs-2250R-A350?

07 August 2016 1,772 3 View

What is the formula to calculating the lattice parameter or lattice constant of Orthorhombic structure?

20 May 2016 9,494 12 View

What is "flow softening" in metal foming?

I work on metal forming processes and I can't find a clear definition about "flow softening" phenomenon. please guide me.

02 May 2016 4,899 3 View

Can anyone suggest a protocol for quantification of fluorescence intensity of extracellular proteoglycans?

I'm going to quantify proteoglycans in spine. I use Imagej Software. I open the file then define the threshold and choose dark background. Then I choose 5 ROIs and measure integrated density for...

22 April 2016 3,107 3 View

Does anyone know how to export displacement from transient structural into fluent in ansys workbench?

Hello every one. I am tring to export transient structural data into fluent .because system coupling is diverging i decided to do it's work by myself. any help appreciated..

25 November 2015 2,211 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View