Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

Which is the best wavelength to study aerosol optical depth(AOD) related to climate reseach,which criteria must be adopted in choosing the wavelengh?

We are doing a study on impact of aerosol on precipitation. Which satellite data and which wavelength will be the best. or can some one suggest a criteria to be adopted to choose an instrument...

12 February 2021 6,324 3 View

How do we calculate 95% confidence intervals for RMSE?

I have two datasets (measured and modelled). I want to calculate 95% confidence intervals for RMSE. Cany anyone please help me with this? Thank you in advance

04 February 2021 5,439 6 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

Doubt in DFT geometry preparation?

Hi all, I am trying to perform some ab inito DFT calculations using cp2k code. I have a unit cell of 120 atoms and would like to know how to refine or reduce the number of atoms in the unit cell...

20 January 2021 6,024 3 View

Which packages can I use for meta-analysis of RNA-seq data and batch effect removal?

Dear All I have already collected RNA-seq expression profile of different studies issuing plant-microbe interaction. I have got read count files for different samples and genes. Now I want to...

14 January 2021 9,119 2 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

Response Surface Methodology?

I'm using 3 different light intensities (constant values 15,30,45 W) for the wastewater treatment of an organic compound and optimizing the parameters (pH, catalyst dose and light intensity)...

31 December 2020 2,377 4 View

What is the impact of Pandemic on the higher education faculty?

Work life Balance, Stress, Anxiety, Effective teaching

28 December 2020 6,201 3 View

Explicit or implicit instruction: The trade-off between feasibility and productivity. Can a language be taught efficiently in an EFL setting?

Teaching realistically in an EFL setting would force us to rely on leaners' explicit knowledge and in turn explicit instruction. On the other hand, long hours of teaching explicitly may not lead...

17 December 2020 9,317 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

How to do variation partition for a distance matrix for microbial ecology?

I have bacterial, fungal, and protist OTU table. I want to know to what extent protist and fungi distribution can explain the bacteria distribution. I only know Mantel test could do a correlation...

11 January 2021 4,164 2 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Why below-ground ecology is still unexplored (majority of it) in our country ?

Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among...

02 January 2021 6,912 3 View

Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View

Shortcomings or consequences of biological synthesis of silver nanoparticles?

Here I want to ask about the shortcomings and consequences of the biological synthesis of nanomaterials. Especially plant and microbial synthesis. Suggest me some reference paper. Thank you

20 December 2020 8,987 3 View

How much the microbial exploration in oil and gas industry is applicable?

Microbial oil and gas prospecting (MPOG) I would grateful it if you could provide me with your invaluable comments and ideas on the following questions: 1- Do you consider MPOG as a promising...

15 December 2020 9,895 2 View

What density do we best consider an experimental unit as a missing plot in Soybean and groundnut. Can someone help share related journals?

Many field experiments particularly for crop plants faces challenges from sowing to germination to phenology up until yield. Too much a few of these challenges includes birds infestation,...

18 October 2020 7,436 4 View

Bioinformatic tools to determine ploidy in microbial genomes?

Looking for tools with proved accuracy to detect ploidy levels in microbial whole-genomes.

14 October 2020 4,273 3 View