How to conclude from the total AKT level, analyzed from western blotting?

hello all, I have done western on A549 cell protein lysate. .I found the total AKT in the treated group was less compared to the control group. How to conclude from this result? many thanks

03 April 2019 8,132 5 View

Which gene expression should I check by qPCR for confirmation of apoptosis?

i am working on an anti cancer drug application on human lungs cancer cell lines (A549). i would like to do preliminary qPCR assay before WB. i am confused of selecting detection of the required...

02 March 2018 4,831 6 View

Primers were specific for the gene of some viruses but not working for other samples. any suggestions?

I am amplifying a specific region of virus genome (650bp) with degenerative primers. my primer set, worked for 6 samples but failed for other other 28 samples. I used 55C the annealing temp. I...

08 September 2017 1,548 3 View

Is there any easiest method to differentiate lactobacillus and Bifidobacterium , if they are co-cultured?

02 March 2016 4,903 6 View

How can i use dialysis membrane for purifying Fructo-oligosacharides?

i am purifying FOS from crude sample, and i want sugars ranging between 3K-8K dalton. below 8K, is easy to obtain, but how to eliminate above things? Thanks

31 December 2015 3,388 1 View

I am planning for my oligo sachharide separation from a plant source, i have chosen sephadex G-100 , what solvent usually works well with G-100?

i have activated my beads with 0.3 M NaCl. Further i could not find any lead.

10 November 2015 2,538 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View