How to conclude from the total AKT level, analyzed from western blotting?

hello all, I have done western on A549 cell protein lysate. .I found the total AKT in the treated group was less compared to the control group. How to conclude from this result? many thanks

03 April 2019 8,132 5 View

Which gene expression should I check by qPCR for confirmation of apoptosis?

i am working on an anti cancer drug application on human lungs cancer cell lines (A549). i would like to do preliminary qPCR assay before WB. i am confused of selecting detection of the required...

02 March 2018 4,831 6 View

How can i finalize optimum amount of cells neded to be used for CCK- 8 ?

I want to check the cytotoxic effect of my treatment on the cancer cells by CCK-8. bu how would j finalize the exact amount of cells to consider in 24 well plates?

11 December 2017 7,095 1 View

Primers were specific for the gene of some viruses but not working for other samples. any suggestions?

I am amplifying a specific region of virus genome (650bp) with degenerative primers. my primer set, worked for 6 samples but failed for other other 28 samples. I used 55C the annealing temp. I...

08 September 2017 1,548 3 View

Is there any easiest method to differentiate lactobacillus and Bifidobacterium , if they are co-cultured?

02 March 2016 4,903 6 View

How can i use dialysis membrane for purifying Fructo-oligosacharides?

i am purifying FOS from crude sample, and i want sugars ranging between 3K-8K dalton. below 8K, is easy to obtain, but how to eliminate above things? Thanks

31 December 2015 3,388 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View