Kishoree Krishna Kumaree

7 Questions 4 Answers 0 Followers

Questions related from Kishoree Krishna Kumaree

How to conclude from the total AKT level, analyzed from western blotting?

hello all, I have done western on A549 cell protein lysate. .I found the total AKT in the treated group was less compared to the control group. How to conclude from this result? many thanks

04 April 2019 8,193 5 View

Which gene expression should I check by qPCR for confirmation of apoptosis?

i am working on an anti cancer drug application on human lungs cancer cell lines (A549). i would like to do preliminary qPCR assay before WB. i am confused of selecting detection of the required...

03 March 2018 4,881 6 View

How can i finalize optimum amount of cells neded to be used for CCK- 8 ?

I want to check the cytotoxic effect of my treatment on the cancer cells by CCK-8. bu how would j finalize the exact amount of cells to consider in 24 well plates?

12 December 2017 7,143 1 View

Primers were specific for the gene of some viruses but not working for other samples. any suggestions?

I am amplifying a specific region of virus genome (650bp) with degenerative primers. my primer set, worked for 6 samples but failed for other other 28 samples. I used 55C the annealing temp. I...

09 September 2017 1,588 3 View

Is there any easiest method to differentiate lactobacillus and Bifidobacterium , if they are co-cultured?

I would like to know, if I do a co-culture of lactic bacteria with Bifido, can they be differentiated on the basis of any bio-chemical test? as PCR can easily differentiate them, but any other...

03 March 2016 4,943 6 View

How can i use dialysis membrane for purifying Fructo-oligosacharides?

i am purifying FOS from crude sample, and i want sugars ranging between 3K-8K dalton. below 8K, is easy to obtain, but how to eliminate above things? Thanks

01 January 2016 3,432 1 View

I am planning for my oligo sachharide separation from a plant source, i have chosen sephadex G-100 , what solvent usually works well with G-100?

i have activated my beads with 0.3 M NaCl. Further i could not find any lead.

11 November 2015 2,593 3 View