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Questions related from Kishoree Krishna Kumaree
hello all, I have done western on A549 cell protein lysate. .I found the total AKT in the treated group was less compared to the control group. How to conclude from this result? many thanks
04 April 2019 8,198 5 View
i am working on an anti cancer drug application on human lungs cancer cell lines (A549). i would like to do preliminary qPCR assay before WB. i am confused of selecting detection of the required...
03 March 2018 4,883 6 View
I want to check the cytotoxic effect of my treatment on the cancer cells by CCK-8. bu how would j finalize the exact amount of cells to consider in 24 well plates?
12 December 2017 7,146 1 View
I am amplifying a specific region of virus genome (650bp) with degenerative primers. my primer set, worked for 6 samples but failed for other other 28 samples. I used 55C the annealing temp. I...
09 September 2017 1,593 3 View
I would like to know, if I do a co-culture of lactic bacteria with Bifido, can they be differentiated on the basis of any bio-chemical test? as PCR can easily differentiate them, but any other...
03 March 2016 4,945 6 View
i am purifying FOS from crude sample, and i want sugars ranging between 3K-8K dalton. below 8K, is easy to obtain, but how to eliminate above things? Thanks
01 January 2016 3,436 1 View
i have activated my beads with 0.3 M NaCl. Further i could not find any lead.
11 November 2015 2,599 3 View