I'm using TRIzol Reagent, but, I think that I have some problems with the homogenize the tissue of pepper leaves (which are minced with liquid nitrogen and stored at -20°C) with the TRIzol, because when I visualize the RNA in an agarose gel, I don't see anything (neither degraded RNA), or perhaps the issue is something that I'm not taking into account.
I really appreciate your advices.
Thank you!
Try to isolate RNA immediately after LN/grinding ("mincing"? Not sure what you're doing. I used to freeze in LN and grind in a pre-chilled (on dry ice) mortar/pestle (different kinds of plants). I don't recommend storing anything for RNA at -20oC. I don't recommend -80oC either. Make your RNA fresh, analyze quickly, and, if necessary, store at -80oC. Let us know how it goes.
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