Diana Marcela Rivera-Toro

6 Questions 3 Answers 0 Followers

Questions related from Diana Marcela Rivera-Toro

PmiREN website works?

Hi Everyone! Does anyone know if the Plant miRNA Encyclopedia database (PmiREN, www.pmiren.com) website has changed? Since the link that has always been published no longer works. Thank you :)

13 September 2023 2,707 0 View

Is LR Clonase an ATP-dependant enzyme?

Hi everyone. I would like to know if anyone knows a way to improve a recombination reaction using the Gateway™ LR Clonase™ II Enzyme mix. I was thinking in adding ATP, but I don't know if the...

20 September 2021 5,634 1 View

Does anyone know of an efficient method for scarifying tomato (micro Tom) seeds?

Hi! I'm having problems wity my tomato (micro tom) seeds, I am doing a manual scarification process, but it is not very efficient in terms of the time it takes to do it for one seed. So I would...

31 March 2021 1,370 4 View

What is the best RNAse inhibitor?

Hello Everyone! I need to send some RNA samples to sequencing, and I want to know which RNAse inhibitor do you use to ensure that your samples don't degrade during shipping and how do you use it...

25 October 2018 2,876 6 View

Which method can I use to build a phylogenetic tree with a capsid protein sequence of a potyvirus in Mega 7?

I'm working with the aminoacid sequence of a capsid protein of a potyvirus and I want to know which method in Mega 7 is appropiate for a phylogenetic analysis Thank you!

01 July 2018 9,097 3 View

How can I extract Total RNA from pepper leaves?

I'm using TRIzol Reagent, but, I think that I have some problems with the homogenize the tissue of pepper leaves (which are minced with liquid nitrogen and stored at -20°C) with the TRIzol,...

01 January 1970 4,028 2 View