HPLC: how to calculate violaxanthin area if it overalps with neoxanthin?

Does anybody know of, or have a method suitable for HPLC analysis that allows to separate violaxanthin from neoxanthin? I want to quantify the xanthophyll cycle (violaxanthin, antheraxanthin and...

21 January 2021 5,775 5 View

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07 December 2020 6,382 2 View

How does the His-Link Protein Purification Resin (Promega) perform?

We have always used Probond (Invitrogen) but Promega resin is cheaper. I was wondering how the Promega resin performs, to see if it is worth to change brands. Thank you!!

18 October 2020 4,351 3 View

How should I approach a Multilevel (2-1-1) Mediation Model with Categorical IV in SPSS or R?

Hi, In my study I have to test a mediation hypothesis in a multilevel (2-1-1) non-longitudinal model, with a categorical IV. I have access to R and SPSS, eventually to MPlus as well. As I...

30 January 2020 2,111 2 View

How do Lexico-semantics features of Nigerian English affect secondary school students spoken English?

What are lexico semantics features of Nigerian English and how do they affect the spoken English of secondary school students?

28 January 2020 9,712 2 View

Can anyone help me with the detection of amyloid beta by western blot?

Can anyone help me with a working protocol for amyloid beta detection by western blot? I have extracted the whole proteins from the cells. For the Western blot I used a Precast gel 4-20% (in SDS...

25 November 2019 3,217 4 View

How to open pictures taken by using InCell2200 (file xdce) in ImageJ?

Hi everybody, I have problems in opening a .xdce file. The file is supposed to contain several series of images taken with the microscope InCell 2200. I tried to open the file by using either...

03 September 2019 4,096 2 View

Does the CellROX dye detect intracellular H2o2 or ROS in general?

Hi, I am looking for a dye to detect H2o2 in living cells that is not fluorecent in green because my cells are already GFP positives. Does anyone have any suggestion? I found the CellRox dye but I...

04 October 2018 4,770 3 View

How can i properly select the solvent to perform an extraction or to prepare a column?

I would like to attach a carbon triple bond to a benzaldehyde ring and to do that i am trying to perform a williamson reaction: https://en.wikipedia.org/wiki/Williamson_ether_synthesis. The two OH...

11 September 2018 4,206 3 View

Gene sequencing to check a SNP?

Hello, I have two cells lines: A375 and Mel-ST. This last one is an immortalized cell line from primary melanocytes. The BRAF gene should be mutated with V600E mutation in A375 cells but not in...

21 June 2018 8,561 6 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View