I have carried out Affymetrix data analysis with oligo and limma. Now I need to perform the gene enrichment analysis on the upregulated and downregulated genes (on EnrichR, by searching the gene symbols ). However, when I annotated my data (with the clariomshumantranscriptcluster.db library as I am 100% sure that the data belongs to human cells) and found the corresponding gene symbol for each probe ID a lot of ID's gave "NA" values.
I have tried to use DAVID and the Affymetrix.com conversion tool but both give no results. I am very confused after reading this on Affymetrix.com: "Annotations beginning with "TC" refer to the TIGR Mouse Gene Index. Annotations beginning with "HT" (Human) or "ET" (other species) are sequence IDs from The Expressed Gene Anatomy Database (EGAD)." because the ID's that I have are all different, I have some starting with "TC", some starting with "HT" and some that are just a number.
I am not sure if I am doing the query search wrong by selecting the wrong GeneChip or by selecting the wrong NetAffx search; or if I am supposed to carry out 3 different searches after separating the different ID formats between HT, TC and number.