How can I extract or isolate an antibody that is water soluble?

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Why are the steppes not the same in Silvaco output?

In Silvaco, I have defined a semiconductor component. The electric field outputs have two problems. One is that the steps of X are not the same and they change, although I have defined a...

19 March 2024 4,649 0 View

How to write the input file for the band structure and optical properties calculation for CASTEP code?

The CASTEP in my case runs without materials studio. I want to calculate the band structure and optical properties of a perovskite material in this CASTEP code. I want to know how to write the...

28 February 2024 5,694 2 View

Did you read any articles about mental imagery for the recovery of stroke patients who used this method for recovery?

Improvement through visualization

22 November 2023 5,807 0 View

How can I ensure the purity of my DNA for PCR?

I'm facing challenges with the purity of plasmid DNA extracted from Trypanosoma brucei for PCR applications. Despite using a commercial DNA extraction kit, I suspect the presence of contaminants...

20 October 2023 829 3 View

What is the best definition of "3D material" in Silvaco?

Dear Sir or Madam I simulated a 3D PIN photodiode in SILVACO ATLAS. It stops in "material" and has an error: " Memory allocation failure. Try killing processes or see system administrator". There...

29 March 2023 7,621 3 View

How can we identify electron affinity, effective conduction or valance band density of states and relative permittivity from DFT data?

I have done DFT calculation of a perovskite material (KGeI3) with CASTEP code. I want to find out its electron affinity, effective conduction or valance band density of states and relative...

17 March 2023 5,912 0 View

HPLC Chromatogram of Benserazide hydrochloride (in both working standard and sample) has two peaks instead one. How can I solve this problem?

This is not contamination. I've used different source of standards and samples and nothing has changed.

23 August 2021 5,460 3 View

Does anyone know anything about the disollution of PEEK polymer in any solvent except sulfuric acid? (the pristine PEEK not sulfonated PEEK)?

Due to the high melting point of PEEK particles, this polymer is insoluble in many solvents except sulfuric acid. I just wants to know that which solvent can dissolve this polymer. Also, I am so...

06 July 2021 7,467 2 View

How can I measure the optical path difference with a quadriwave lateral shearing wavefront sensor and a microscope?

Hi, I am trying to measure the change of the refractive index (RI) of my sample by measuring the OPD with a QWLS wavefront senor. For this aim I have two different setups. My first setup is the...

06 April 2021 8,185 4 View

If i have a model and the constant is insignificant, do i need to let the variable or remove it from the model?

I am going to check the affect of Budget deficit on Economic growth and My Model is ARDL. I have to say when I remove constant from model then other tests will reject the model. Best Regard.

08 January 2021 3,439 7 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View