Dear Md. Rafat Tahsin

You can extract alkaloids by following these methods.

Alkaloid Extraction from Powdered Leaf (General Method)

1. Sample Preparation

• Use air-dried, powdered leaves.

2. Extraction

• Soxhlet extraction with methanol or ethanol for 6–8 hours (or cold maceration for 48–72 hrs with occasional shaking)

3. Concentration

• Evaporate solvent using rotary evaporator to get crude extract.

4. Alkaloid Isolation (Acid-Base Method)

5. Purification

• Use column chromatography or TLC for isolation.
• Confirm with HPLC/NMR/MS if needed.

Thanks.

To extract Carbazole-Alkaloids or Terpenoid-Alkaloids from powdered plant leaves start with a solvent extraction method. First, dry and powder the plant leaves finely. Use methanol or ethanol as a solvent to macerate or reflux the powdered material for several hours to extract the alkaloids. After filtration, concentrate the extract using a rotary evaporator. Then, perform acid-base extraction: acidify the concentrated extract with dilute HCl to protonate the alkaloids (making them water-soluble) extract non-alkaloid impurities with organic solvents (like hexane or chloroform) then basify the aqueous layer with ammonia or NaOH to liberate the free alkaloids. Extract these with chloroform or dichloromethane. Finally, evaporate the solvent to obtain the crude alkaloid mixture. Further purification can be done using column chromatography or HPLC depending on the specific compound.