You can either centrifuge it between 5000 to 10,000 rpm for 10 minutes at four degrees or filter it with 0.2 micron syringe filter. Do not use 100% conditioned media. It will kill your cells. You have to titrate it for optimal volume, for example 10% conditioned medium and 90% culture medium.

Thanks Piyush, This is what we usually does before storing them. But I am not sure whether this high speed centrifugation or 0.2 micro filtration would be enough to remove Licl/ BIO particles from the CM. Please share if you have any literature details here.

What I am thinking right now is to go for a couple of ultracentrifugation steps to isolate exosomes out of it. That also possibly will serve my purpose.