Hi all,
I have performed CRISPR-cas9 on my cell line and seeded them at 0.5 cell per well.
To screen for frameshifted clones, i want to duplicate my plate, so i will have two: one to screen using NGS and one to save the clone(i'll freeze that plate).
Does anyone know a better/less time consuming method than trypsinizing all wells and seeding them 50/50?
Thanks a lot in advance!