Erik van Butselaar

4 Questions 7 Answers 0 Followers

Questions related from Erik van Butselaar

How can i duplicate a 96 well plate easily?

Hi all, I have performed CRISPR-cas9 on my cell line and seeded them at 0.5 cell per well. To screen for frameshifted clones, i want to duplicate my plate, so i will have two: one to screen...

24 October 2017 4,689 2 View

How can i duplicate a 96 well plate easily?

Hi all, I have performed CRISPR-cas9 on my cell line and seeded them at 0.5 cell per well. To screen for frameshifted clones, i want to duplicate my plate, so i will have two: one to screen...

10 October 2017 1,181 3 View

Is it possible to isolate nuclear fraction of cells for RNA analysis?

I'm currently investigating a lncRNA and i'm very curious to see what the subcellular location is due to silencing strategy and prediction of lncRNA function. Could i somehow separate the nucleus...

27 June 2017 4,324 3 View

Is it possible to isolate nuclear fraction of cells for RNA analysis?

I'm currently investigating a lncRNA and i'm very curious to see what the subcellular location is due to silencing strategy and prediction of lncRNA function. Could i somehow separate the nucleus...

06 June 2017 3,279 3 View