I'm currently investigating a lncRNA and i'm very curious to see what the subcellular location is due to silencing strategy and prediction of lncRNA function. Could i somehow separate the nucleus from cytoplasm and isolate/qPCR to find out where expression is present?
I was looking at fractioning protocols, but these are mostly aimed at proteins, if at all possible, could you recommend me a protocol for RNA analysis?
Huge thanks in advance!
Hi Noor Edan Jarad,
Thank you for your reply.
Unfortunately the link refers back to the homepage of thermofisher. if you still have it available, could you resend it?
To answer my own question for if someone is looking for it:
This paper i found describes their protocol very well and i will use it as pilot
https://www.ncbi.nlm.nih.gov/pubmed/16893657
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA