I have an H2O2 biosensor electrode and I want to optimize the experiment, such as the pH of the PBS and applied voltage of the sensor. So how can I do it? And should the PBS solution stir during the optimization to find the current response or not? The current sensitivity influence by the pH value of the test PBS solution, applied potential, and concentration of enzyme. These experimental variables need to optimize in order to achieve a sensitive biosensor. So which technique can carry out this optimization to find the optimum value for the applied potential and pH, is it amperometric or CV? And if amperometric, the PBS solution should be stirred?
Hello team at Rayan Engg.
Could you please elaborate your actual experimental procedure to help us know what exactly is being done and what needs to be added?
Thanks!
If I read this correctly, you have 4 things that can affect your outcome, right?
If that is true, you can try using a 12-run Plackett Burman Design. This will give you an idea about which combinations of factors affect your outcome. From there, you can optimize your response.
You can use Response Surface Methodology to optimize your input parameters.
@ Masood, could you elaborate your explanation? You got my point from my question?!
Yes indeed. You wanted to convey that, a lot of literature is available on internet.
EAch piece of equipment is different. That means each piece of equipment has a different "optimal" setting. The only way to find it is to test combinations of your settings and see what gives you the best results. A PB Design or Response Surface will help get you there. Usually, one would do a PB design first to see which direction is optimal then use a response surface as a final step.
If you have four significant parameters and each one has four levels, then things are going to be different.
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