I work on ethanol production from agricultural wastes. I need to determine the content of ethanol production in media content more carbohydrates by chemical method I not use Gas chromotgrphy method or GC
Dichromate oxidation method is used for ethanol determination.
Caputi A Jr, Ueda M, Brown T (1968) Spectrophotometric determination of ethanol in wine. Am. J. Enol. Vitic. 19: 160-165.
See this paper:
Ka¨gi JHR, Vallee BL. 1960. The role of zinc in alcohol dehydrogenase. V.
The effect of metal-binding agents on the structure of the yeast alcohol
dehydrogenase molecule. Biol Chem 235:3188–3192.
find attachment for determination f ethanol .
Caputi A, Ueda M T Brown T (1968),“Spectrophotometric determination of ethanol in wine”, American Journal of Enol. Vitic 19:L160-165.
If you have the ethanol the only compoenet in your solution you may use refractive index for it
Lucas test is used to identify/estimate secondary and tertiary alcohols. But, the question is about ethanol, which is a primary alcohol. Lucas test gives negative result for primary alcohols. Hence, Lucas test cannot be used for estimation of ethanol.
A method for measuring the ethanol concentration in a yeast culture broth was developed using both microtubes and a 96-deepwell microplate. The strategy involved the solvent extraction of ethanol and measurement the ethanol concentration by using the dichromate oxidation method. The article has been attached.
- Badges
- Science topic