Using AMPure XP Beads if possible.
Do you have any protocol or idea of the beads proportion I have to use ?
Thanks
I thought the beads were useful to separate larger fragments from small MW contaminants and primers etc. But not so useful to size separate in that limited size range.
Hello Maxime,
This could give you some info on size selection chemistry with beads, they cite two core papers on the subject.
http://seqanswers.com/forums/showthread.php?t=17887
In my opinion, to achieve a double size selection you should need two consecutive selections: the first one with 'higher' PEG, so you precipitate fragments < 2Kb, and the second one with 'lower' PEG, so you precipitate fragments >1.5 Kb. Whether size selection can be so sensitive I'm not sure, and I would expect substantially low efficiencies when doing two steps.
If you can't achieve the goal with the beads with your expected efficiency, I would do gel extraction and excise the desired range, and then purify with a method of choice.
Best,
Ruben
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