How to describe exposure variables?

Hello, I would like to review the literature on the intrinsic and extrinsic risks to women that can negatively influence their childbirth experience. I have a difficulty in developing my research...

01 December 2020 5,781 3 View

What is the best protocol for simultaneous extraction and purification of DNA and RNA from animal tissues?

I need to perform a co-extraction of DNA and RNA from pools of juvenile Daphnia magna. I used the AllPrep DNA/RNA Mini Kit (QIAGEN) but with poor results in terms of DNA yeld and quality. I would...

22 October 2020 9,746 3 View

How to find compounds that are similar to others in term of structure and activity?

Dear all, I Have found in silico two compounds that are interesting for me, one is spermidine (a metabolite) and another one is negamycin. Negamycin has apparently an effect on my cellular models...

20 October 2020 8,394 3 View

A good reference for polyphasic identification of Acremonium-like species?

I am wondering if there is a good book or web reference for identification of species from the genus Acremonium and its likes.

31 August 2020 1,878 2 View

Checking the independent errors assumption for logistic regression in SPSS?

I am investigating the effect of certain cognitions/attitudes on sexual recidivism. Sexual recidivism is the binary outcome variable (0 = no / 1 = yes). Originally the sex offenders could score 0...

07 May 2019 7,295 6 View

No title

21 October 2018 1,995 1 View

Differences between the files "rna_from_genomic" and "rna" ?

Hi ! I was wondering what were the differences between those three files : [https://www.ncbi.nlm.nih.gov/genome/doc/ftpfaq/] *_rna.fna.gz : FASTA format of accessioned RNA products annotated on...

24 December 2017 3,117 2 View

Is it possible to enrich/selectively isolate poly-dT+ cDNA?

Is it possible to enrich/selectively isolate poly-dT+ cDNA from a sample of cDNA which was reverse transcribed from total RNA using a mix of oligo-dT and random primers?

29 January 2017 9,080 5 View

Interference in SDS-PAGE silver staining of peanut extract?

I'm currently working on the optimization of the trypsin digestion protocol (duration and trypsin concentration) of peanut extracts (with Tris and Urea). I use SDS PAGE (12% BioRad) to check the...

23 November 2016 5,534 4 View

Abaqus: General contact and coupled thermal-electrical-structural step N/A?

I'm building a vibrothermography model with a coupled electro-thermal-mechanical step, where a piezoelectric actuator generates ultrasonic waves that are transmitted to a material, where the...

24 August 2016 692 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View