Ruben Alvarez-Fernandez

5 Questions 70 Answers 0 Followers

Questions related from Ruben Alvarez-Fernandez

How is primer annealing and optimal concentration influenced by 5' dangling ends in PCR?

Dear all, Just wondering about the effect of very long 5' dangling ends (>60bp) on PCR in the first few cycles. An obvious thing is that after extending them once, they are effectively not...

08 February 2016 9,857 10 View

Could someone give me insight into why gene duplications could be occurring when targeting essential genes in S. pombe?

We are trying to make conditional mutants in essential genes of Schizosaccharomyces pombe, using the nmt1 promoter and different degrons, and antibiotic selection in haploid cells. The homologous...

22 October 2015 1,755 3 View

How to retrieve a probe from a CATMA microarray slide?

Hi all, We have done experiments (a timeseries, which we used to model expression profiles) with the CATMA array platform. We found a gene that looked quite interesting, and there were two probes...

11 May 2015 2,415 2 View

How can opposite perturbations in gene expression generate the same same phenotype?

Hi all, I have been giving thought to this, and I was wondering if someone knows about literature that tackles this phenomenon? Thing is, I have arabidopsis plants that lack a protein (due to a...

19 February 2015 8,503 4 View

Does anyone have the pBDGAL4 plasmid?

This plasmid is an old one for yeast two-hybrid work, but I am trying to find it to set up a practical. It has been discontinued from the original source, Agilent, and I have not found it in...

26 September 2014 4,260 5 View