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Dear all, Just wondering about the effect of very long 5' dangling ends (>60bp) on PCR in the first few cycles. An obvious thing is that after extending them once, they are effectively not...
08 February 2016 9,814 10 View
We are trying to make conditional mutants in essential genes of Schizosaccharomyces pombe, using the nmt1 promoter and different degrons, and antibiotic selection in haploid cells. The homologous...
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Hi all, We have done experiments (a timeseries, which we used to model expression profiles) with the CATMA array platform. We found a gene that looked quite interesting, and there were two probes...
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This plasmid is an old one for yeast two-hybrid work, but I am trying to find it to set up a practical. It has been discontinued from the original source, Agilent, and I have not found it in...
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