How can I distinguish the antigen of antibody : folding protein or unfolding protein?

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Ramachandramouli Budida Popular answer

You can check whether your antibody binds to the unfolded antigen, you can try western blotting, which denatures the protein to linear form.

Ramachandramouli Budida

Alemu Tekewe Mogus

I agree with the comments given by Budida. Antibodies can recognize unfolded antigens if their target epitopes have a linear/or a non-conformational structure. You can test this using western blot under denaturing conditions as suggested by Budida.