When drawing a phylogenetic tree with somatic mutations(SNPs), what is the role of tree simulation process?

Hello, I am working on drawing a phylogenetic tree with samples sharing some somatic mutations. The basic process of my work is similar to this article. Population dynamics of normal human blood...

14 October 2020 8,078 4 View

In grignard reaction, how can I attach 'Mg' to only one side of dibromoalkane?

As you can see in the image file, I want to make Br-C12-dithiocarboxylic acid form. However, I don't know how to control the amounts of reactant. I tried 'Mg:alkane=1:1 equiv' but many byproducts...

18 September 2020 3,970 4 View

I would like to mix FBS and horse serum (1:1) when culturing cells, do you think it would change sequencing results?

Hello, I am doing fibroblast, stem cell culture for sequencing. (From primary culture to the end) Since FBS is expensive, there are some protocols that mix FBS and horse serum (1:1) I have done...

10 September 2020 8,137 3 View

Is there any way I can do spheroid culture with fibroblasts (not cancer cell)?

I have done spheroid culture with various cancer cell lines The spheroid was formed from single cells. The method was like the added picture (not curved bottom well, matrigel, etc) The question...

30 March 2020 9,817 3 View

Why use dol dme electrolyte in lithium sulfur batteries mostly?

In lithium metal battery with transition metal cathode, they use lipf6 salt and different electrolyte. However, in lithium sulfur batteries, almost studies were conducted under dol dme electrolyte...

30 March 2020 9,603 4 View

Some of SH-SY5Y cells detach during media exchange in 96well How can I deal with?

Hi all, I seeded 1 X 10^4 of SH-SY5Y on 96 well plate and grew them overnight. For drug experiment, I removed all media using pipette and treated media mixed with drug. However, I found that some...

23 March 2020 8,924 4 View

Would you let me know how to extract Polysaccharides?

I'm planning to extract Polysaccharide from Codium fragile, which is green algae. I used to use hot water for extraction for 3 hours and then use Ethanol to get them. I tried this way, but It...

17 September 2019 4,558 5 View

About photonic crystal, Is there any conditions that minimum number of polymer layers of particle which can color?

I understand that incresing number of layers causes high reflectance..but I don't know the minimum condition that particle can be used as photonic crystal..

08 August 2019 1,413 1 View

Vimentin Western Blot: No consistency of the change in vimentin expression level of A549 cell line. What can I do?

I am working on Epithelial to Mesenchymal Transition (EMT) in A549 cell line by treating positive inducers such as EGF to up-regulate the mesenchymal markers like vimentin. I'm doing western blot...

22 May 2019 3,053 1 View

Can I use this live/dead cell assay kit after I fixed the samples?

https://www.thermofisher.com/order/catalog/product/L3224 This is the live/dead cell assay kit I will use for my experiment. But I have to fix samples (cancer cells) before using this kit, maybe...

29 April 2019 3,481 3 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View

How can I remove deep eutectic solvents from the medium?

Dear researchers, How can I remove deep eutectic solvents from the medium? You know, generally, deep eutectic solvents consist of ammonium salts and an acid group. Is there any alternative method...

22 February 2021 3,275 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

When I reacted Bidentate NS Schiff Base with metal salt (M-Cl2) in +2 oxidation state in proportion of (2:1) , getting sticky product ,, why?

When I reacted Bidentate NS Schiff Base with metal salt (M-Cl2) metal chlorides in +2 oxidation state in proportion of (2:1 Schiff base &metal salt) , i was get sticky material Why ? ---~...

21 February 2021 7,970 5 View