Hello,

Carbohydrates, lipids and proteins were extracted from the same sample according to the procedure of Shibko et al. (1966), while that of glycogen according to Van Handel (1965). The dosage of the carbohydrates and glycogen was realized according to the method of Duchateau & Florkin (1959). The lipids were given according to the method of Goldsworthy et al. (1972). Lastly, the proteins were quantified according to the method of Bradford (1976).

Plesae fing the following refrences concerning the protocols.

 BRADFORD M.M., 1976.- A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analyt. Biochem., 72. 248-254.

DUCHATEAU. G, FLOKIN. M. 1959. Sur la tréhalosémie des insectes et sa signification. Archives of Insect Biochemistry and Physiology 67: 306-314.

GOLDSWORTHY, G.H., MORDUE, W. & GUTHKELCH, J. (1972).- Studies on insect adipokinetic hormones. Gen. Comp. Endocrinol., 18, 545-551.

SHIBKO, S., KOIVISTOINEN, P., TRATYNECK, C., NEW HALL, A. & FREIDMAN, L. (1966).- A method for the sequential quantative separation and glycogen from a single rat liver homogenate or from a subcellular fraction. Anal. Biochem., 19, 415-428.

Van HANDEL E., 1965. Microseparation of glycogen, sugars and lipids, Analytical Biochemistry   Analyt. Biochem. 11: 266-271.

You can find in reserchagte noureddine soltani some publications dealing with the dosage of main biochemical components in several mosquito species.

Good luck Pr. N. Soltani

Dear colleague,

I think that the protocols correspond to your question and are satisfactory for your research.

Best wihes Pr. N. Soltani

Thank you so much Prof. Soltani. It was quite helpful. 

Best regards

dear Colleague,

If you agree with my answer you can up voted it.

Thanks 

Best regards Pr. N. Soltani

Hi Azubuike, 

Attached is the protocol I used to my metabolism analysis. I hope this help and let me know if you have any questions.