Hello,
Does anyone have any idea of the exact (if any) number of cells we can have in mosquito salivary glands (the species doesnt matter at this stage).
Thank you
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?
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If the pairs are similar, will it reduce the adhesion due to electric charges?
29 July 2024 3,185 2 View
I've been trying to design primers for qPCR to detect NCF1 cDNA using Primer3Plus and NCBI Primer-BLAST, but I couldn't find primers that are specific to the gene. Any help ? Thanks !
04 July 2024 3,068 3 View
I am trying to understand the influence of polymer films sliding against each other. For the simulation, I saw many molecular dynamics simulations, like LAMMPS, GROMACS, etc. I am new to this...
09 April 2024 8,871 2 View
I have been trying for months to grow kidney cells from rat kidneys, I tried several methods, serial sieving, cutting and meshing, and using kidney tissue to obtain an outgrowth of cells. I always...
11 March 2024 2,665 1 View
if I have 5 grams of PMMA, how much (mL) of Chloroform should I used to dissolve it ? should U use sealed beaker to this process?
25 February 2024 9,066 0 View
Hi how can I upload a manuscript for a paper that wasn't uploaded before thanks?
21 December 2023 7,777 0 View
Hi, I will be culturing BM-MSCs in DMEM culture media, based on the literature, I will add 10% FBS and pen/strep... except when I checked the one that we have, I found that we only have...
10 December 2023 6,681 3 View
Hello, I have been reviewing several articles on DNA methylation quantification for aging studies. I don't want to do a complete genome DNA methylation quantification, since it is quite costly,...
02 November 2023 3,006 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View