7 Questions 15 Answers 0 Followers
Questions related from Syed Raza
I am studying the migration of nanoparticles in a 72hours chick embryo model. However I am not sure about the diameter of the capillaries of 72 hours chick embryo. I want to start with...
04 April 2016 1,559 2 View
Are there any specific markers for recognizing the stages of Alzheimer (mild, severe etc) in rodents. I wish to induce the same using streptozotocin, and transplant stem cells both at an early and...
04 April 2016 7,224 3 View
Hi, I am quite new to the plasmid selection guide. I wish to select a GFP plasmid for lentiviral assembly to finally transfect mouse cell, and also for an another study: shSOD1 expression plasmid...
03 March 2015 4,778 6 View
How can (What are the techniques?) I detect a frameshift mutation in both the alleles of a gene induced by my CRISPR-Lentivirus? Please suggest. Syed
03 March 2015 10,098 2 View
Hi All, I am trying to transduce my NPCs with the mCherry lentivirus, but I am getting very few transduce cells (Red). My NPCs are already GFP transducer, herewith I am attaching few pictures...
02 February 2015 2,171 19 View
Hi All, A friend of mine from India has raised a concern since they don't have the transduction facility, what are the other ways to trace the transplanted Stem Cells in vivo in rodents if they...
02 February 2015 3,849 16 View
Do anyone have an idea what is the basis of taking ratio of DNA/envelope/packaging for Lentivirus preparation? My target vector is of 14kb but I don't know what rail should I take for my 2nd...
01 January 2015 6,330 1 View
