How do different stages of alzheimer can be recognize in rodents?

Are there any specific markers for recognizing the stages of Alzheimer (mild, severe etc) in rodents. I wish to induce the same using streptozotocin, and transplant stem cells both at an early and...

03 April 2016 7,179 3 View

What is the dimeter of the 72h chick embryo capillaries?

03 April 2016 1,517 2 View

How should I select an expression plasmid for a lentivirus construct?

Hi, I am quite new to the plasmid selection guide. I wish to select a GFP plasmid for lentiviral assembly to finally transfect mouse cell, and also for an another study: shSOD1 expression plasmid...

02 March 2015 4,717 6 View

How can I detect a frameshift mutation in both the alleles of a gene induced by my CRISPR-Lentivirus?

02 March 2015 9,990 2 View

How can I trace transplanted Stem Cells in vivo, which are not labelled for any color?

01 February 2015 3,779 16 View

How do I solve my difficulties with neural progenitor stem cells lentiviral transduction?

Hi All, I am trying to transduce my NPCs with the mCherry lentivirus, but I am getting very few transduce cells (Red). My NPCs are already GFP transducer, herewith I am attaching few pictures...

01 February 2015 2,118 19 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View