20 April 2022 3 640 Report

I am a beginner in the field of Polymerase spiral reaction primer design. What are the most important considerations when developing primers for the Polymerase spiral reaction?

Thank you in advance for your insightful advice.

More Gyanendra Singh Sengar's questions See All
How can the collaboration between the state and cooperative sector be enhanced?

The partnership between the government and cooperative sector is crucial for fostering equitable national development. While the government may not have the reach into every sector, the private...

17 January 2024 8,158 2 View

What is code for single value prediction through ANN for regressor problem?

I am designing the ANN code for my dataset (which is actually regressor type problem having 3 IDV and 1 DV).I am getting a good accuracy but i couldn't find the code to predict any single value...

18 July 2023 1,024 3 View

How to apply Artificial neural network in the dataset with 3 independent variable and 1 dependent variable to predict any value through model?

I have actually 3 independent variable and 1 dependent variable i want to predict the unknown data that i need .I have done it with regression but i am facing errors in ANN.

11 July 2023 2,297 5 View

How to predict data with 3 independent variable and 30,000 dependent variable by using machine learning algorithm??

I have 3 independent variable which is height(100,300,500),angle(0,30,......,180) and pressure point(1,2.......,112) and each pressure point has 30,000 values of wind pressure coefficient,I want...

11 July 2023 1,400 12 View

How can we seed cells insitu on algiante-tyramine hydrogels crosslinked via HRP and H2O2?

I need a brief protocol regarding seeding of cells in situ with alginate-tyramine crosslinked hydrogel and taking that cells for Q RT-PCR study after 72 hours. please mention if there is any...

13 November 2022 5,077 2 View

How to perform RNA isolation and RT-PCR for Cells seeded in 3d hydrogels ?

I want to do RNA isolation and RT-PCR of cells seeded on 3d hydrogels with high purity and noninterference of hydrogels. Can you please suggest a detailed protocol to achieve the same?

27 October 2022 2,072 3 View

Purification of fluorescent labelled bisphosphonate ?

Can anyone share the protocol for purification of any fluorescent labelled bisphosphonate drug other than RP-HPLC?

25 February 2022 5,864 6 View

How to mitigate the numerical cherenkov radiation/instability in CST Studio suite?

I am trying to simulate the electron bunch dynamics in the particle in cell (PIC) solver of CST Studio suite, but I observe numerical Cherenkov radiation in the electron bunch (for high bunch...

03 February 2022 6,942 1 View

Does absorption oscillate due to interference?

In UV-VIS analysis of a thin film, suppose we measure Reflectance and absorbance. Absorbance sometimes can be different in meaning. Some instruments include the reflectance in the absorbance, and...

15 April 2021 3,715 3 View

What are the different bagging algorithms other than Random Forests and Extra Trees Classifier?

Please give the names of different bagging algorithms. I know only Random Forest and Extra Trees.

25 January 2021 1,185 5 View

Similar questions and discussions
I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How to Input and Analyze Results for an Asymmetrical Factorial Design in STATISTICA 13.3?

In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...

29 July 2024 4,155 2 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close