I want to do RNA isolation and RT-PCR of cells seeded on 3d hydrogels with high purity and noninterference of hydrogels. Can you please suggest a detailed protocol to achieve the same?
RNA extraction from the cells cultured in a hydrogel mainly depends on the hydrogel's nature and composition. It can be done by dissolving the hydrogel and then taking out the cells for RNA isolation. Here are some papers that discussed some methods. you may need to standardize according to your hydrogel system.
Article RNA extraction from self-assembling peptide hydrogels to all...
Article Techniques for the Isolation of High-Quality RNA from Cells ...
Article Techniques for RNA extraction from cells cultured in starPEG...
Saurabh Mandal Thank you for your suggestion. I will read the articles. However,I would like to add one more question i.e, Can you also suggest when you are adding an enzyme for dissolving hydrogels and when you are adding Trizol for RNA isolation?
Without knowing that with what your hydrogel is made up of, it will be difficult for members to give any right suggestions. I don't work extensively but am exposed to peptide-based and sodium alginate hydrogel. For sodium alginate, we used a decapsulation solution consisting of EDTA, HEPES, and PBS. we reported that in our paper.
Article Encapsulated human mesenchymal stem cells (eMSCs) as a novel...
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