I need a brief protocol regarding seeding of cells in situ with alginate-tyramine crosslinked hydrogel and taking that cells for Q RT-PCR study after 72 hours. please mention if there is any method regarding the same?
Article Macroporous 3D printed structures for regenerative medicine ...
Dmitriy Berillo thank you
How to build a heterostructure in VESTA/VASP of two pristine materials having different space group and structure?
27 July 2024 739 2 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
Any Upcoming call for papers in journals (SCI/SCIE indexed) related to image processing or computer vision. Special issues related to my area, whose submission dates are within a few months.
23 June 2024 5,021 1 View
Electron back scattered diffraction is a versatile tool for micro-structure and micro-texture analysis of a variety of materials e.g., metals/alloys, rocks, meteorites etc., EBSD completely works...
18 June 2024 1,477 5 View
I am working on a new polymer whose (hkl) values are not reported in the literature. How can I find out the (hkl) values of the XRD peaks?
17 June 2024 820 5 View
I have prepared some polymeric microspheres from chitosan and PCL. However while performing in vitro degradation study, I found difficulties in both above mentioned methods. Here I am mentioning...
20 May 2024 6,114 4 View
Hello guys, I am optimizing a perovskite photodetector device with the device structure ITO/PTAA/Cs0.15FA0.85PbI3/PCBM/BCP/Ag. PTAA (2 mg/mL): 5000 rpm/30 sec Perovskite (1.3 M): 1000 rpm/10...
10 May 2024 2,297 0 View
What is the mechanism behind increase in the peak intensity with filler loading in polymer nanocomposite. In my work, the (014) peak corresponding to the filler increased proportionately with...
08 May 2024 7,972 3 View
Hello guys, What might be the reason for getting two PL peaks after deposition of PCBM onto Cs0.15FA0.85PbI3 perovskite?
18 April 2024 5,169 0 View
Dear all, My device structure is ITO/PEDOT:PSS/Cs0.15FA0.85PbI3/PCBM/PEI/Ag. But till now, I am getting Jsc nearly 13. So please suggest to me how I can increase my Jsc and why I can not achieve...
13 March 2024 9,601 1 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...
28 July 2024 2,794 6 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...
20 July 2024 3,611 4 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.
11 July 2024 9,045 4 View