Regouat Nadia

10 Questions 7 Answers 0 Followers

Questions related from Regouat Nadia

Hello, please, any one can suggest me an other reagent to substitute ''gold chlorid'' in order to perform ''reticulin fiber stainning''?

I want to perfom ''reticulin fiber stainning'' using silver impregnation technique according to ''Gordon et Sweets., 1936. In the last step of the staining, i need to use ''gold chlorid''. it's...

02 February 2025 4,044 1 View

Hello, what is the best way to dissolve tamoxifen citrate?

I need to disolve tamoxifen citrate tablets without using ethanol, is it possible to use corn oil or DMSO like with tamoxifen.

04 January 2022 7,733 4 View

Can I replace o-dianisidine dihydrocholride with o-dianisidine in the ceruloplasmin assay?

I want to measure ceruloplasmin according to Schoslnsky, 1974. I tried o-dianisidine to replace o-dianisidine dihydrocholride but it did not work, Can I try it again with a more acid pH acetate...

03 April 2017 6,947 1 View

Please how to proceed to find the appropriate dilution of an antibody for use in the rat?

the recommended dilution in human 1: 200

01 February 2017 5,350 2 View

Can anyone give me informations for measurement of pyruvate and lactate on heart homogenate?

is there a method for measurmnet of lactate and pyruvate on heart homogenate

02 October 2016 498 0 View

Is there anyone who can give me details of the preparation and the technical protocol of hematoxylin basic fuschin picric acid staining ?

I want tu use this staining to detect eventual domages in cardiomyocytes.

07 February 2016 9,631 4 View

Can I use the BRADFFORT method for assaying proteins in the myocardium?

I want to quantify myocardial proteins level using a simple technique . Is the BRADFORD method's good for detecting the maximum of protein at this level ?

15 January 2016 8,144 3 View

What is the normal concentration of testosterone and estradiol in male rats?

Please, i want to substitute adult male rats after castration with testosterone and oestradiol, at what concentration i must do it, how often per day?

07 June 2015 7,456 10 View

How can I correct a handling error ?

Please, During the preparation of the fixative for electron microscopy I made a mistake, I forgot to dilute gluteraldehyde before adding it, I left my samples about an hour, then I catch and I...

04 June 2015 8,979 3 View

Please, how I can make a compatage of smooth muscle cells on a histological section of the aorta ?

comptage of smooth muscle celle

21 May 2015 6,657 1 View