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02 March 2021 932 1 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Solution of 2D convection diffusion heat equation using MATLAB ?

This is a polymer heating problem. Polymer enters at around 180C (x=0) in the barrel. Also barrel initial temperature is 180 (t=0). Some heat Is added along whole length of barrel q. We have to...

15 February 2021 3,887 1 View

How is Initial GDP measured ?

Dear All, I am working on estimating Debt-Growth Threshold but confused about some points. My questions are; - How do we calculate initial GDP Per Capita? Shall we do it in STATA or excel file?...

15 February 2021 2,677 2 View

What is the magnetic anisotropy constant for Permalloy (NiFe-80-20)? Can anyone suggest some papers?

Need to know the equation of anisotropy energy for permalloy and also the constants. Any reference paper will be helpful. Thanks for your time and cooperation.

26 January 2021 7,842 3 View

Similarity between these two waveform? Similarity in terms of Shape?

Hi, These are the outputs generated by a system. Green-colored (nr) is desired output, while the system generates Red-colored (nf). I also have a dataset in CSV file. For this waveform, What...

21 January 2021 929 2 View

What are the future research avenues in context of Urban Street, Outdoor thermal comfort and Neural Networks?

Dear academic community, I am a young researcher from architectural background. I am looking for possible novel approaches to evaluate outdoor thermal comfort in urban streets using artificial...

20 January 2021 7,964 3 View

Can we control the position and vibration, simultaneously, of a flexible link with a tip mass using only the actuator's input?

This is a boundary control problem of a hybrid ODE-PDE system. The system consists of an actuator, a flexible link fixed to the actuator, and a tip mass at the free end of the link. In most...

13 January 2021 8,050 2 View

What is the vital connection of the constitution in global Governance?

I want to connect a legal affinity and directive formalization between Constitutional Government and global Governance in my research paper.

03 January 2021 9,023 1 View

Hi dear fellow researchers and senior scientists ,i need validate method for Safranin-O-fast green images intensity quantification?

Dear fellows i have images of meniscus sections stained with Safranin-O-fast green. I need to quantify the images for Safranin-O-fast green intensity and any other parameter if...

30 December 2020 1,931 3 View

ISSR Annealing Temperature Calculator, which formula can I use?

I was wondering if I can use a formula for my ISSR primers to calculate the annealing temperature and melting temperature. Alternatively I would like to know if is possible to calculate with any...

27 October 2020 8,885 3 View

May anyone help me to insert binary data into Popgene correctly?

Hello everyone, I am trying to use Popgene 32 software for analysis of binary data for dominant marker of five ISSR primers across five populations and having 38 loci. Unfortunately the program...

22 September 2020 7,369 4 View

I have to score the gel images for ISSR markers. Could you please explain how scoring the gel images for ISSR markers?

I have to score the gel images generated by ISSR markers. Could you please explain how scoring the gel images for ISSR markers?

08 July 2020 5,944 2 View

Is there something I can do to increase the chances of successful ISSR bands amplification during PCR ?

Hello, I have a following issue: I extracted approx. 20 plant DNA samples from dry leaves (old herbarium items) using Qiagen DNeasy Plant Mini kit. When I run a PCR for housekeeping gene (alpha...

22 March 2020 2,664 6 View

How to calculate distance matrix between taxa using binary (0 ,1) data For ISSR markers?

Dear Professors, I have binary data (0,1) for ISSR markers and i need to construct a dendrogram to relate five taxa. How can i calculate distance matrix, how to draw a dendrogram manually, which...

01 February 2020 8,179 3 View

What ISSR primers will Best be used for genetic profiling of plants in astereceae family like vernonia amygdalina?

I have used RAPD for profiling and got little or no bands, now I want to try ISSR primers. So I am seeking to know if anybody has done similar work and the primers used.

07 August 2019 2,097 3 View

How do I improve band visualization of PCR?

I am testing ISSR primers on plant DNA and the bands I get are very faint. I am adding 5 microliters to the wells from a 25 microliter PCR product, I have tried increasing the volume in the well...

09 February 2019 1,477 6 View

How do I improve band visualization of PCR?

I am testing ISSR primers on plant DNA and the bands I get are very faint. I am adding 5 microliters to the wells from a 25 microliter PCR product, I have tried increasing the volume in the well...

01 February 2019 8,333 7 View

How can I prepare cladogram using binary data of RAPD ana ISSR?

I have the sofwares, MEGA, TnT etc.but couldn't managed to prepare commands to input my binary data which is in excel format. Please guide me to prepare the cladogram.

11 December 2018 3,216 7 View

Can the same primers be used for ISSR AND SSR?

I WANT TO KNOW IF THE SEQUENCE OF PRIMERS USED FOR ISSR CAN ALSO BE USED FOR SSR FOR A PARTICULAR PLANT SPECIE LIKE VERNONIA AMYGDALINA

08 August 2018 6,374 4 View