Why some of my DNA samples are not working with ISSRs?

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When doing antibody titration for flow cytometry, is the unstained control sufficient as a negative control, or isotype control also required?

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What is the best country/university for studying synthetic biology?

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What is the best way to ship tissue samples internationally that are already preserved in EtOH?

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What are the differences between mathematical modelling and realistic mathematic education?

what are the differences between mathematical modelling and realistic mathematic education?

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How do I increase the yield of my PCR product?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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MiRNA qpcr problem?

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Runs of nucleotides in Sanger sequencing?

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Can thermal cycler machines be problematic during PCR?

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Calculating volume of DNA required from DNA concentration?

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Has anyone used the Illumina ITS1 primer sets?

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Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

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Ricardo Julian Licea-Moreno

Dear Emel Ören have you checked the quality of DNA of those samples that don't work? Have you considered the posibility that this marker don't work with some strain(s)?

Rasmieh Hamid

Have you tested your DNA reproducibility with PCR of an internal gene?

M. A. El-Sayed

Dear Emel Oren

checke the quality of DNA

Emel Ören

Thank you all. ITS1-4 worked just fine wt the samples that did not work with ISSR.