Can anyone tell me how to calculate the Genetic diversity (Nei) from gel images?

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Gizella Jahnke

For RAPD markers, the genetic background is not always known, so if you want to calculate some similarity index from gel images, I suggest scoring the bands as present or absent. The resulting data can be used to calculate e.g. a Jaccard index. If you need software support, I recommend MolMarker.

Reference: https://www.mdpi.com/1424-2818/14/6/497

Avaiable at: https://sourceforge.net/projects/molmarker/

Sreeram Sudhir

Thank you mam!

Shamsunnahar Mukta

If you have the scoring, it's better to run some software analysis for diversity. You can check NTSYSpc, POPGENE, Mol Marker, Power marker.