Importance of distance education?

Distance learning is a learning method in which students are not required to be physically present in a classroom setting or school.

25 December 2020 8,494 10 View

Difference on natural farming and organic farming???

Based on my perception, organic farming we can use organic based fertilizers like biofertilizers, Azolla, Panchakavya, Neem oil extract etc. Whereas, in natural farming no application of any kind...

23 November 2020 6,890 22 View

What is the exact sequence for Lac Z alpha gene including promoter/operator/terminator?

Dear Sir/Mam, I am constructing vector/plasmid with Lac Z alpha gene. I have doubt the following sequence is correct to insert Lac Zalpha-...

30 March 2018 1,075 1 View

How to solve mixed level design in Minitab 17?

To Solve mixed level design with 3 factors and factor 1(6 level), factor 2(5level), factor3(4-level), I have used Minitab general design Full factorial -with 2 replications, totally 240...

01 February 2017 9,282 9 View

What is the best way to calibrate an XRF? Use Certified reference materials as Primary standards or use secondary standards ?

What is the best way to calibrate XRF which will be used to test chemistry of Clays and Feldspars? Is going for Certified Reference Materials from companies like Fisher Scientic, MBH Analytical...

19 June 2016 3,058 2 View

How to quantify results in HPLC UV detector or any formula applying?

How can quantify in the results of HPLC per ml. e.x result 10 mg/ml.or any formula apply to quantify compound.?

13 April 2016 698 3 View

I am new to molecular works - I am getting more non-specific bands in my PCR product, how can I prevent this?

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05 March 2013 8,664 19 View

Cardiac isoenzyme expression by SDS-PAGE

I need a help, please any one suggest the procedure for the electrophoretic separation of creatine kinase isoenzymes in serum.

05 September 2011 3,973 1 View

Which tool is good for to draw the phylogeny using morphological characters?

01 January 1970 3,845 7 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Can downdraft gasifier for RDF be scaled up?

Dear researchers, In literature, so far I am seeing that downdraft is usually used for small-scale purposes (10kW-10MW). One of the reasons behind is "they do not allow for uniform distribution...

08 February 2021 5,403 1 View

How to calculate radial distribution function (RDF) of a complex from Ab initio MD simulation trajectory?

Hi all, Is there any software or webserver to read the molecular dynamics (MD) simulation trajectory which obtained from Ab initio MD simulation to calculate the atom-atom radial distribution...

25 January 2021 9,474 3 View

Which is best tool for alignment of large bacterial sequence?

Hi! I need to align big fasta files (bacterial 16S) . I have tried to align them in some web-servers and freeware, but my files are too big to be run in there. I will be very glad if you could...

16 January 2021 1,266 4 View

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

How does the gmx rdf will act if we consider multiple molecules for -ref?

I need to calculate the radial distribution function for my system which includes 50 molecules. Here is my code line to generate rdf in gromacs: gmx rdf -n Index.ndx -f mdrun1.xtc -s mdrun1.tpr...

04 January 2021 4,673 8 View

How to interpret data in .arp file?

Hey, I am working in population genetics. I used DNAsp to convert fasta into .arp file for input in Arlequin. Can anyone help to interpret the data in the .arp file? As in which column represents...

12 October 2020 8,443 9 View

How to interpret the RDF plots?

I have studied the structural changes of a protein in 3 different concentrations of ionic liquids (50 mM, 500 mM, and 1M). On analysis of the RDF plots, I find that the magnitude of the RDF plot...

05 October 2020 4,972 2 View

Surface RDF in Gromacs?

Hello, I am trying to use the -surf option in gmx rdf in order to compute the rdf of certain molecules from the surface (nearest atoms) of my reference molecule . My script is: gmx rdf -s...

22 September 2020 5,097 10 View