Where could I purchase whole cells with 5-HT (serotonin) 1B receptors?

Hi! I would like to study 5-HT 1B (Serotonin 1B) receptors and conduct a Gi CAMP assay on it. As you know, this is a functional assay that requires whole cells and not membrane preparations. Do...

19 January 2021 3,614 2 View

Any leads on open-source RI libraries?

Hi! Does anyone know any libraries where we can access retention index data (gas chromatography) of aroma compounds for free? Thanks!

07 December 2020 1,951 1 View

Is European Journal of Education Studies a good journal for publication of an article from a dissertation?

I sent an article to the journal and the reviewers indicated that the work can be published as it is. there were no suggested corrections and this left me wondering if its a recommendable journal....

19 May 2020 8,131 3 View

Is it okay to use Technical/Laboratory Grade solvent for extraction and open column chromatography?

Some would say that the process of separation through the use of high-grade silica and other chromatographic materials will eventually result to separation of impurities so it's okay to use...

12 January 2020 9,987 5 View

How do you improve peak separation in this case (Please see attached picture) ?

How do I improve the peak shape and resolution in this region of the chromatogram? Analytes are volatiles in the headspace of rice. Column is DB-Wax-- a column reported to be successful in...

24 November 2019 7,250 3 View

Why are there broad, irregularly-shaped peaks that elute early in my gas chromatogram ?

We just replaced our GC-FID with a new column and conditioned the column before using it. When I started my running blanks and samples using a specific temperature program, there always appears...

13 November 2019 9,378 3 View

Why are there fewer peaks in a gas chromatogram with longer run times than with shorter run times?

Lengthening my GC run time from 14 min to 34 min resulted in fewer peaks. The target compound that I am trying to quantify is not detected whenever I run my samples (same extraction conditions)...

08 October 2019 7,512 6 View

Can anyone recommend a good SEC-HPLC column that can be used with peptides at pH 2.5?

I am intend to buy a SEC-HPLC column that is suitable to used with the peptide Glucagon(29 amino acids) at a pH=2.5. Can anyone recommend a column that might be suitable? The company, APEX, that...

29 August 2019 6,027 1 View

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18 July 2019 5,164 3 View

What are suitable peptides/proteins to build a calibration curve for SEC-HPLC in a low molecular weight range?

I want to do HPLC-SEC that characterizes the low-order oligomers for the low molecular weight peptide Glucagon(Mol.Wt.=3.48kDa). Does anyone know suitable reference peptides/proteins that could...

21 May 2019 7,637 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View