How can I interpret the values of zero-sequence impedance in the pic. and use them for short circuit current calculus in a 3winding power transformer?

I have a three winding power transformer with the zero-sequence impedance values given in the picture. I need to model those in a equivalent circuit for simulation purposes.

23 December 2020 3,866 1 View

How to Interpret Box Plot IQR?

I have a unidimensional marital satisfaction scale (Johnson, Zabriskie, & Hill, 2006) with five items. My sample was 194 married individuals, and using the data from my sample, I wanted to...

29 September 2020 6,595 3 View

How can i dissolve curcumin in ethanol (% of ethanol?) for antimicrobial test ?

I want to test the antimicrobial properties of curcumin, is ethanol the best solvent? if yes what is the least percent concentration i can use to dissolve the curcumin powder?

21 September 2020 5,890 5 View

Do you think that schools could resume quickly after the lockdown period due to the emphasis on social distancing, following the Covid-19 pandemic?

It may be difficult for schools to resume immediately after the lockdown period as a result of the emphasis on social distancing rules, following the Covid-19 pandemic?

27 April 2020 9,766 96 View

How does one compute energy eigenvalues in atomic unit?

please find attached question Thank you

20 April 2020 4,025 9 View

How to take advantage of the social isolation generated by COVID-19 to boost the transition from face-to-face education to remote education?

The isolation generated by the COVID-19 pandemic has forced most of the world's universities to choose remote or virtual classes. In the case of engineering programs and other programs where real...

28 March 2020 2,350 57 View

In which powder bed fusion equipments have been implemented the skywriting scan strategy?

25 March 2020 8,679 4 View

Is it possible to reduce energy consumption without affecting human well-being?

Climate change is a reality. Responsible for this change is the excessive concentration of CO2 in the atmosphere, mainly due to the requirement of electricity at the residential, commercial,...

13 February 2020 8,805 12 View

How to estimate charge transfer and polarization resistance in a battery?

Hi. I have performed many EIS experiments using a cylindrical battery at different temperatures and state of charge. Some results (near SOC=0) are as the attached figure. How can I find the...

08 July 2019 1,746 2 View

Anyone experienced in using Dab library from Daniel Christ?

I have been troubleshooting the use of this library for the isolation of antibody fragments for a couple of months with some success.However I went into some troubleshooting. I'm looking for...

16 June 2019 8,389 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

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17 February 2021 4,217 3 View